Enhanced Expression of Recombinant Activin A in Escherichia coli by Optimization of Induction Parameters

Advances and challenges in recombinant protein expression in Escherichia coli

The Expression of Human Granulocyte Macrophage Colony Stimulating Factor by Heat-Induction in Escherichia coli

A self-regulated high-copy number plasmid containing chloramphenicol resistant gene, for the production of recombinant proteins under the regulation of bacteriophage ?pL promoter, was constructed. The designed 5024 base pair expression plasmid contained a heat sensitive repressor cI857 coding gene to regulate the function of ?pL promoter under heat shock induction. Using the constructed vector,...

optimization of auto-induction conditions for the heterologous expression of a maltogenic amylase in escherichia coli

background and objectives: auto-induction is usually employed to achieve high cell density and overproduction of proteins with a simple and low-cost operation. the efficiency of heterologous protein expression in escherichia coli is determined by different parameters. interactions between these parameters usually complicate the identification of those that contribute more to the improvement of ...

Soluble Expression of Recombinant Nerve Growth Factor in Cytoplasm of Escherichia coli

Background: Pivotal roles of Nerve growth factor (NGF) in the development and survival of both neuronal and non-neuronal cells indicate its potential for the treatment of neurodegenerative diseases. However, investigation of NGF deficits in different diseases requires the availability of properly folded human b-NGF. In previous studies bacterial expression of hNGF demonstrated the feasibility o...

Optimization of Cloning Conditions for high-level Production of Recombinant Mouse Interleukin-2 in Escherichia coli

Backgrounds and objectives: Interleukin 2 (IL-2) secreted by activated CD4+ T cells  has been known as a major mediator in both  adaptive and native immune system  due to a board range of effects on  different cells in the immunity system (1-6). Methods: cDNA synthesis was performed using gene- specific primers designed by Gene Runner software after RNA extraction of mouse splenocytes. PCR pro...

Enhanced Production of Insulin-Like Growth Factor I Protein in Escherichia coli by optimization of five key factors

Abstract Human insulin-like growth factor I (hIGF-I) is a kind of growth factor with clinical significance in medicine. The major objective of this study is over- production of recombinant human insulin-like growth factor I( rhIGF-I) through a developed process by recruiting effective factors in order to achieve the most recombinant protein. Up to now E. coli expression system has been widely us...